Simultaneous Chloramphenicol and Florfenicol Determination by A Validated DLLME-HPLC-UV Method in Pasteurized Milk
نویسندگان
چکیده مقاله:
The antibiotic residues in milk are a well-known serious problem and pose several health hazards to consumers. We have described a simple, rapid, and inexpensive DLLME-HPLC/UV technique for the extraction of chloramphenicol and florfenicol residues in milk samples. Under the optimum conditions, linearity of the method was observed over the range 0.02-0.85 µg/L with correlation coefficients > 0.999. The proposed method has been found to have a good limit of detection (signal to noise ratio = 3) for chloramphenicol (12.5 µg/kg) and florfenicol (12.2 µg/kg), and precision with relative standard deviation values under 15% (RSD, n = 3). Good recoveries (69.1–79.4 %) were obtained for the extraction of the target analytes in milk samples. This simple and economic method has been applied for analyses of 15 real milk samples. Among all samples only one of them was contaminated to florfenicol; 62.4 µg/kg and contamination to chloramphenicol was not detected.
منابع مشابه
simultaneous chloramphenicol and florfenicol determination by a validated dllme-hplc-uv method in pasteurized milk
the antibiotic residues in milk are a well-known serious problem and pose several health hazards to consumers. we have described a simple, rapid, and inexpensive dllme-hplc/uv technique for the extraction of chloramphenicol and florfenicol residues in milk samples. under the optimum conditions, linearity of the method was observed over the range 0.02-0.85 µg/l with correlation coefficients > 0....
متن کاملA Validated Simultaneous Rp-hplc Method
A new RP-HPLC method was developed for selective and simultaneous determination of of Desogestrel and Ethinyl Estradiol Tablets. The chromatographic separation was achieved on a Zorbax SB C-18, 4.6 x 250mm, and 5μ column. The gradient LC method employs solutions A and B as mobile phase. The solution A milliQ water and acetonitrile in the ratio of 90:10 and degas and solution B contains a mixtur...
متن کاملExtraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method
Novel dispersive liquid-liquid microextraction (DLLME), coupled with high performance liquid chromatography with photodiode array detection (HPLC-DAD) has been applied for the extraction and determination of cyproheptadine (CPH), an antihistamine, in human urine samples. In this method, 0.6 mL of acetonitrile (disperser solvent) containing 30 µL of carbon tetrachloride (extraction solvent) was ...
متن کاملExtraction and Determination of Cyproheptadine in Human Urine by DLLME-HPLC Method
Novel dispersive liquid-liquid microextraction (DLLME), coupled with high performance liquid chromatography with photodiode array detection (HPLC-DAD) has been applied for the extraction and determination of cyproheptadine (CPH), an antihistamine, in human urine samples. In this method, 0.6 mL of acetonitrile (disperser solvent) containing 30 µL of carbon tetrachloride (extraction solvent) was ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ذخیره در منابع من قبلا به منابع من ذحیره شده{@ msg_add @}
عنوان ژورنال
دوره 15 شماره 3
صفحات 361- 368
تاریخ انتشار 2016-10-01
با دنبال کردن یک ژورنال هنگامی که شماره جدید این ژورنال منتشر می شود به شما از طریق ایمیل اطلاع داده می شود.
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023